Jian-Jian Yu, Jian-Ge Han, Yi Tan, Jian-Xiang Xu, Amanda LeBlanc, Bradley B. Keller, Jiapeng Huang, Lu Cai
University of Louisville School of Medicine. Tianjin University Chest Hospital. Cincinnati Children’s Heart Institute and University of Cincinnati.
United States and China
World Journal of Diabetes
World J Diabetes 2025; 13:
DOI: 10.4239/wjd.v16.i10.109526
Abstract
Background: Diabetes has become a widespread metabolic disease affecting multiple organs. Among diabetic complications, cardiovascular complications are the main cause of patient morbidity and mortality. Diabetic cardiomyopathy is a diabetes-specific cardiomyopathy in the absence of other cardiovascular disease and occurs more frequently in type 1 diabetes (T1D) than in type 2 diabetes. Previous studies on diabetic cardiomyopathy have predominantly focused on the effects of diabetes on left ventricular (LV) dysfunction, while studies of right ventricular (RV) dysfunction have been sparse but are gaining attention. Although T1D accounts for only 5%-10% of the total diabetic population, diabetic cardiomyopathy is a major cause of morbidity and mortality in children with life-long, long-term complications.
Aim: To evaluate longitudinal RV and LV functional changes in female transgenic OVE26, T1D mice and wild-type FVB mice over a 30-week period.
Methods: RV and LV structure and function were evaluated by transthoracic echocardiography. RV systolic pressure was measured by a transducer-tipped pressure catheter. Sirius-red staining was used to quantify collagen and fibrosis, wheat germ agglutinin staining was utilized to measure cardiomyocyte size, and quantitative real-time polymerase chain reaction and Western blotting were used to quantify miRNA expression and protein abundance, respectively.
Results: RV systolic function, measured by tricuspid valve annular plane systolic excursion and RV systolic velocity, was similar between control and T1D mice, but LV systolic function decreased in T1D mice at 30 weeks of age. RV diastolic dysfunction in T1D mice significantly increased by 18 weeks and progressed until 30 weeks, while LV diastolic dysfunction trended towards abnormal at 12 weeks, significantly increased by 18 weeks, and continued to progress by 30 weeks. Furthermore, RV diastolic dysfunction was accompanied by RV cardiac fibrosis and hypertrophy in T1D mice, occurring later than that in the LV. Pulmonary arterial hypertension developed in T1D mice, evidenced by increased pulmonary acceleration time to pulmonary ejection time ratio and increased RV peak systolic pressure at 30 weeks. These results suggest the development of early LV diastolic dysfunction followed by LV systolic dysfunction and RV diastolic dysfunction at 30 weeks in T1D mice.
Conclusion: RV diastolic dysfunction develops later than LV dysfunction in OVE26 T1D mice. Mild pulmonary arterial hypertension appear at later stages of T1D and could contribute to RV systolic impairment and remodeling.
Category
Class II. Pulmonary Hypertension Associated with Left Ventricular Systolic or Diastolic Dysfunction
Heart Dysfunction Associated with Pulmonary Vascular Disease (Right and Left)
Animal Models of Pulmonary Vascular Disease and Therapy
Age Focus: No Age-Related Focus
Fresh or Filed Publication: Fresh (PHresh). Less than 1-2 years since publication
Article Access
Free PDF File or Full Text Article Available Through PubMed or DOI: Yes